Product Information (ASFV Antigen Test Strip)
| Kit components | Volume |
| African Swine Fever Virus Antigen Test Strip (including disposable dropper) | 1 strip per bag, 25 bags per box |
| Sample buffer | 300µL per tube, 25 tubes per box |
| Disposable cotton swabs | 25 pieces per box |
| Instruction Manual | 1 copy per box |
Test Procedure
Usage
1.1 Samples of serum, blood and anticoagulated blood
Use a dropper to absorb the whole blood, serum or anticoagulated blood sample, and drip one drop into the sample well. Then add two drops of sample buffer solution. After the sample loading is completed, place the test card flat on the desktop. Observe the results within 5 to 10 minutes. Results obtained after 15 minutes are invalid.
1.2 Swab samples
Use a cotton swab to collect the nasal and oral fluid or fecal samples of the pigs to be tested. Immediately insert the sampled cotton swab into the sample buffer solution tube, stir for 10 to 15 seconds and then take it out. After covering the tube cap, turn it over 8 to 10 times and let it stand for 1 minute to allow the undissolved samples to settle. The processed sample is the specimen to be tested. (Note: Excessive fecal sampling amount will affect the stability of colloidal gold particles and is likely to cause false positives. The sample amount should preferably cover 1/3 to 2/3 of the cotton swab tip.)
1.2.1 Take out the test card bag and tear it open (use one bag for one sample), take out the test card and place it horizontally on the operating platform (do not pick it up from the platform during the testing process, and it can be picked up only after the testing is completed).
1.2.2 Use a dropper to absorb the specimen to be tested, and vertically drip three drops into the sample well of the test card, and then start timing.
1.2.3 Read the results within 5 to 10 minutes. Results obtained after 15 minutes are invalid.
Judgment
2.1 Positive (+): A band appears at the position of the C line and a band also appears at the position of the T line, indicating that the African swine fever virus antigen is contained in the sample.
2.2 Negative (-): A band appears at the position of the C line while no band appears at the position of the T line, indicating that the African swine fever virus antigen is not contained in the sample.
2.3 Invalid: No band appears at the position of the C line, indicating that the operation process is incorrect or the test strip has become invalid.